The ELx™ is a compact, robust microplate reader ideally Software, the ELx applications are expanded to include kinetic Absorbance Test Plate. Shop online for a wide selection of BioTek ELx Absorbance Microplate Readers Store up to 75 custom assay definitions and eight test results. 1 Introducing the ELx Absorbance Microplate Reader. and provides the means for downloading additional assay definition files to the instrument.
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The TS Absorbance Reader offers both affordability and proven performance for microplate-based assays in in a variety of research and clinical applications. The ELx™ Absorbance Microplate Reader offers proven performance for many applications, from endpoint to fast kinetic protocols. The ELx is widely. ELx Absorbance Microplate Reader with computer software is in our new catalogue Features.
How can I learn more about the MVS? It includes equipment, software, Sample Solutions and characterized microtiter plates to allow operators to quickly and easily verify the liquid delivery performance for automated liquid handlers and multichannel pipettes within minutes. The MVS also allows for multichannel pipette operators to be assessed for pipetting competency and then trained accordingly. The system is suitable for liquid volume measurements from 0. The MVS is positioned on a mobile workstation to allow for portable, convenient volume verification of pipettors in multiple locations.
Endophytic bacteria that are especially associated with medicinal plants presents a reservoir of therapeutic compounds. Fagonia indica has been recently investigated by numerous researchers because of its striking therapeutic potential especially in cancer. It is also reported that endophytes play a vital role in the biosynthesis of various metabolites; therefore we believe that endophytes associated with F.
The present study aims successful isolation, molecular identification of endophytic bacteria and their screening for bioactive metabolites quantification and in vitro pharmacological activities. Methods 16S rRNA gene sequencing was used for the identification of isolated endophytic bacteria. Methanolic extracts were evaluated for total phenolic contents TPC , total flavonoids contents TFC , DPPH free radical scavenging activity, reducing power and total anti-oxidant assays were performed.
And also screened for antibacterial and antifungal activities by disc diffusion method and their MIC were calculated by broth dilution method using microplate reader. Further, standard protocols were followed for antileishmanial activity and protein kinase inhibition. Results Bacterial strains belonging to various genera Bacillus, Enterobacter, Pantoea, Erwinia and Stenotrophomonas were isolated and identified.
Although all the extracts tested were active to inhibit growth of selected pathogenic microbes bacteria and fungi , but significant antibacterial activity was observed against Klebsiella pneumonia and B.
An Enterobacter cloaca was active against Leishmania tropica with IC50 value of 1. Conclusions Our findings revealed that crude extracts of selected endophytic bacteria from F. Over the years, emergence of new infectious diseases such as Ebola, Swine flu H1N1 , Dengue fever and severe acute respiratory syndrome SARS , have been added to this challenges [ 1 ].
There is a general need for chemotherapeutic agents, antibiotics and pharmaceutical drugs with high effectiveness and low cytotoxicity. New medicinal agents are also needed for nematode infections such as malaria and for the treatment of parasitic protozoan diseases such as leishmaniasis and trypanosomiasis [ 2 ]. Natural resources are the potential source of novel bioactive molecules and can be used in the treatment of various infections.
Such sources may include various forms of life like plants, marine macro-organisms sponge, corals and algae and endophytes fungi, bacteria and actinomycetes [ 3 ]. Plants which are used in traditional medicines are of significant importance and therefore considerable research has been carried out on medicinal plants for bioactive compounds however limited research has been performed on the associated microorganisms.
Endophytes are chemical synthesizer inside plants. They play a role as a selection system for microbes to produce pharmacologically active substances with low toxicity toward mammalians [ 4 ]. Endophytes are sometimes responsible for the medicinal properties of their hosts. Many endophytes synthesize bioactive compounds that can be used by plants for defense against pathogens and some of these compounds may be valuable as pharmaceutical drugs [ 5 ].
Fagonia indica is a small spiny shrub that belongs to the family Zygophyllaceae present in warm and arid regions of the world, especially Asia and Africa [ 6 ]. Fagonia indica is of great ethno pharmacological importance with multiple therapeutic activities such as antimicrobial, antioxidant, antiseptic, anticancer, antidiabetic and anti-inflammatory [ 7 ].
The plant is also used for the treatment of fever, thirst, vomiting, asthma, urinary discharge, dysentery, liver and stomach trouble, toothache, typhoid and skin diseases [ 8 ]. This study was designed to investigate the role of endophytic bacteria in the medicinal properties of F. Bacteria were isolated, identified and screened for the production of bioactive secondary metabolites. Methods Plants collection and identification The plant samples were collected in sample bags from village Mullazai, Peshawar, Khyber PakhtunKhwa, Pakistan and brought to the laboratory at the same day for the isolation of endophytic bacteria.
The plant material was taxonomically identified as F. Herbarium specimen Voucher No. Isolation of endophytic bacteria from stem of Fagonia indica The stem of the plant was cut into pieces of about 0.
Stem pieces 5—6 were blotted on the blotting paper [ 9 ] and placed on selected Tryptic Soy Agar TSA media for the isolation of endophytic bacteria. Molecular identification of isolated endophytic bacteria Bacterial genomic DNA was isolated using alkaline lysis method and 16S rRNA gene was amplified by performing colony PCR using universal bacterial primers 27F and R, which produced a product of size base pairs [ 10 ].
The system components include a customized Plate Reader, a unique Calibrator Plate, a microtiter Plate Shaker, a bar code reader, a portable computer with the MVS Data Manager system software, a mobile workstation and a user guide. On-site system installation and validation are also included with an MVS purchase.
Can I use my own microwell plate reader? No, we do not support the use of other plate readers at this time.
The MVS includes a mobile workstation for convenient verification of equipment in multiple locations throughout a laboratory or facility. Yes, the MVS is a convenient way to verify the accuracy and precision performance of each independent channel at the same time.
The MVS allows for analysis of accuracy and precision for each independent dispensing channel simultaneously, which saves labor and liquid handler down-time. It is relatively immune to environmental conditions and provides measurements traceable to national and international standards. There is no need to generate standard curves.
MVS provides standardized results making it possible to compare instrument performance between makes and models of liquid handlers as well as between locations, i. The MVS results are traceable to national and international standards. The only regular maintenance required is the recertification of the MVS Calibrator Plate, which has a shelf-life of 1-year.
At the one year mark, the Calibrator Plate must be shipped back to Artel for the recertification process. Aside from Calibrator Plate recertification, the only other routine maintenance required is the occasional replacement of the lamp in the Artel Plate Reader dependent on plate reader used , which can be easily completed by the user.
The MVS can be easily used by technicians and scientists of any education level. Artel has done all the work for liquid handler QC to make volume verification fast, easy, and reliable.
MVS Data Manager system software guides the user through the entire volume verification process and all the Sample Solutions are prepared by Artel. The user simply pours the solutions into a reservoir, dispenses the solution with the liquid handler under test, and measures the tip-by-tip volume values using the MVS.
The MVS is used by many different types of laboratories serving many different fields. Basically, it is used wherever laboratories have liquid handlers and wherever they are serious about liquid handling quality control. Customers and laboratories using the MVS include: 1 research and development labs including HTS, compound management, lead optimization ; 2 clinical and reference labs; 3 governmental labs; 4 forensic labs; 5 manufacturers of automated liquid handlers; 6 liquid handling or automation engineers; 7 manufacturing labs; and 8 production support labs.
Concentration of reagents in microtiter plates is volume dependent. If the right volume is not being dispensed, the reagent concentrations could be different as compared to the theoretical values. Without the right concentrations, the assay results might be called into question or could be falsely interpreted.